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Spidermonkey's Sporadic Lab Stuff

by Spidermonkey

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Hello World from the lab!

Part spider - part lab monkey, will do science for cash, strawberries, or for a place to be that's out of the rain (but preferably for cash).

The rest of what I get up to goes into the Tunnel of Goats :)

Urgent Science Stuff:

Please help reform English libel law
Current UK libel laws are very bad for science and free speech.


Cool science stuff:

RCSB Protein data bank: Molecule of the Month in alphabetical order

The PCR Song It's the little things that keep you going...

NCBI's Entrez Gene My favourite starting point for finding out what is known about any gene of interest.

NCBI's PubMed Where to go to find pretty much all research published in the last 30 years or so (may go back a lot further now). All newly published research is quickly added to the site.

The Genetic Code - table of the DNA/RNA triplet codes for amino acids. This is how DNA codes for protein.

Another PCR song Disco frenzy :)


Recent visitors

Oh ELISA

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It's been a while since my last post here, but I'm still doing science where I can. For the last few months I've been volunteering in an MS research lab where I have been mostly running ELISAs - assays that use protein-specific antibodies to measure levels of your favourite protein of the day in your samples, in this case neurofilament light-chain levels in cersbrospinal fluid samples. It's good to keep my hand in and learn some new stuff while I try to find a proper job. I guess you never know where things will lead.
14th Jul 2011, 22:37  

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Dania says:

Oooh, I like this. Pretty.

I actually like running ELISAs, ever since the first time I ran one and saw the whole class getting false positives in all samples except for me and my lab partner who got everything as expected. We felt so lucky competent. :D

I had no idea you also had this moblog. Looks like I'll have to keep an eye on it. :)

14th Jul 2011, 23:52

Dania says:

I see. The strikethrough tag doesn't work here. Oh well. It was supposed to go on "lucky".

14th Jul 2011, 23:56

Spidermonkey says:

Glad you like it. I couldn't get the colours to come out right on the camera at all - it had a real problem with different shades of yellow so this is the less informative but prettier view :)

You must have very green fingers for lab things. Very well done for your lab practical :) While the ELISA process is quite simple it takes a lot of concentration and attention to detail to do them well. I have become even more OCD than usual about my standard curves and pipetting in order to keep things consistent between the different plates. I've run nearly 50 now and am quite relieved that I am at the end of this particular project. On the plus side, I now have a very strong right thumb :)

15th Jul 2011, 07:36

Dania says:

Well, I thought I was going to be a disaster in the lab when I decided to go into biochemistry, because sometimes my hands are a bit shaky. But as it turns out, all I need is to concentrate on what I'm doing and relax, and the shakiness goes away. Not a problem. I think I can say I'm no disaster, after all. :)

Good luck on trying to find a proper job. I suspect I'll be needing it too in the not-so-distant-as-it-seems future... :-/

15th Jul 2011, 16:53

Spidermonkey says:

Ah yes, the 96-well plate zen state :)
Thanks and good luck to you too.

20th Jul 2011, 16:11