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Spidermonkey's Sporadic Lab Stuff

by Spidermonkey

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Hello World from the lab!

Part spider - part lab monkey, will do science for cash, strawberries, or for a place to be that's out of the rain (but preferably for cash).

The rest of what I get up to goes into the Tunnel of Goats :)

Urgent Science Stuff:

Please help reform English libel law
Current UK libel laws are very bad for science and free speech.

Cool science stuff:

RCSB Protein data bank: Molecule of the Month in alphabetical order

The PCR Song It's the little things that keep you going...

NCBI's Entrez Gene My favourite starting point for finding out what is known about any gene of interest.

NCBI's PubMed Where to go to find pretty much all research published in the last 30 years or so (may go back a lot further now). All newly published research is quickly added to the site.

The Genetic Code - table of the DNA/RNA triplet codes for amino acids. This is how DNA codes for protein.

Another PCR song Disco frenzy :)

Recent visitors

The unhappiest PCR in the world (so far)

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With a lot of persuasion the enzyme is just about making the band that I want it too. You can hardly see it, but it is there in the middle 4 lanes. The lanes at either end are ladders of size markers (Pieces of DNA at different sizes). The band I want is just below the position of the second band from the top on the left-hand ladder, directly below the dark circles left by the loading dye (loading dye helps you see a) that you have got your DNA into the gel in the first place and b) how far your DNA has run when the gel is in the tank).

I have cut out the regions where my band is and will extract the DNA from that, then use that as template for another round of PCR to amplify up that specific bit some more. That's the plan.

*edit: just looking at this posted here you really can't see the bands at all except maybe the merest hint of it in the 3rd middle lane (from left to right). You can just about see it on the original and with the naked eye (behind a visor!) and a serious UV light source.
10th Jan 2010, 22:36  


FilbertFox says:

You need the eye of Faith. As she frequently told us.

I can see it. No really I can

15th Jan 2010, 14:42

crickson says:

Loading buffer also tells you c. you switched on the wrong power unit 10 minutes ago and your DNA is now floating freely about the tank, as it did for me this morning.

15th Jan 2010, 16:54

FilbertFox says:

been there done that!

16th Jan 2010, 15:56

itchymoblog says:

I love the abstract art nature of what you do for a living :)

6th Jul 2010, 12:09