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Spidermonkey's Sporadic Lab Stuff

by Spidermonkey

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Hello World from the lab!

Part spider - part lab monkey, will do science for cash, strawberries, or for a place to be that's out of the rain (but preferably for cash).

The rest of what I get up to goes into the Tunnel of Goats :)

Urgent Science Stuff:

Please help reform English libel law
Current UK libel laws are very bad for science and free speech.

Cool science stuff:

RCSB Protein data bank: Molecule of the Month in alphabetical order

The PCR Song It's the little things that keep you going...

NCBI's Entrez Gene My favourite starting point for finding out what is known about any gene of interest.

NCBI's PubMed Where to go to find pretty much all research published in the last 30 years or so (may go back a lot further now). All newly published research is quickly added to the site.

The Genetic Code - table of the DNA/RNA triplet codes for amino acids. This is how DNA codes for protein.

Another PCR song Disco frenzy :)

Recent visitors

These are still not the clones you are looking for

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But they do help me figure out where the problem(s) are. The new ligase is a lot lot better than the old stuff, so at least I know that bit should work. Unfortunately it still isn't working with the pieces I want to stick back together. It is possible there is a mistake somewhere in the sequences used to make the bits of DNA in the first place, or for whatever reason this bit of DNA adopts some structural conformation which makes it stick to itself rather than to where I would like to stick it...
Leaving it here for today.
At least my luciferase assay made a change and has me thinking about something else I don't understand.
Now to go and help in the kitchen!
26th Nov 2009, 16:50  


Caine says:

I'm glad you're getting closer. Fabulous shots!

26th Nov 2009, 23:14

Viv says:

you know this just illustrates how good it is for you to be back at work :)

27th Nov 2009, 09:26