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Spidermonkey's Sporadic Lab Stuff

by Spidermonkey

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Hello World from the lab!

Part spider - part lab monkey, will do science for cash, strawberries, or for a place to be that's out of the rain (but preferably for cash).

The rest of what I get up to goes into the Tunnel of Goats :)

Urgent Science Stuff:

Please help reform English libel law
Current UK libel laws are very bad for science and free speech.

Cool science stuff:

RCSB Protein data bank: Molecule of the Month in alphabetical order

The PCR Song It's the little things that keep you going...

NCBI's Entrez Gene My favourite starting point for finding out what is known about any gene of interest.

NCBI's PubMed Where to go to find pretty much all research published in the last 30 years or so (may go back a lot further now). All newly published research is quickly added to the site.

The Genetic Code - table of the DNA/RNA triplet codes for amino acids. This is how DNA codes for protein.

Another PCR song Disco frenzy :)

Recent visitors

Today i am mostly modelling

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It makes a change from cloning and is something to do while I wait for new ligase to arrive.

Ligase is the enzyme that is supposed to stick my new favourite piece of DNA into the green-fluorescent protein vector so that it forms a circle again. This circle is then what I introduce to the bacterial cells in the hope that they'll take it up and make more of it (like little microbiological factories). Then, when they've made more of it, I extract the circular DNA (but not the bacteria's chromosomal DNA) back from the bugs and transfect it into the liver cells I have going in culture. Then I get to treat those cells with compounds of interest to see whether my favourite protein will bind to it and take it into the nucleus of the cell (the GFP tag means you can see where the proteins are (mostly) in the cell.

None of this has a chance of working if the ligase won't stick the DNA back together. I'm hoping (really, really hoping) new ligase will solve all my problems.

In the meantime, I have a ton of modelling to catch up on.
24th Nov 2009, 01:32   | tags:


Spidermonkey says:

Thanks George :) I'll be happier with it when it's working. Doesn't feel too excellent right now but I'm trying.

24th Nov 2009, 02:37

bethmadethis says:

wow, these look pretty.

24th Nov 2009, 09:19

Spidermonkey says:

If you want to see more pretty protein structures try the RCSB protein data bank. They do a 'molecule of the month' feature which is a really easy way to get into protein structures. They've done some really cool ones. Here's the link to the molecule of the month alphabetical list :)

25th Nov 2009, 04:13