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Spidermonkey's Sporadic Lab Stuff

by Spidermonkey

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Hello World from the lab!

Part spider - part lab monkey, will do science for cash, strawberries, or for a place to be that's out of the rain (but preferably for cash).

The rest of what I get up to goes into the Tunnel of Goats :)

Urgent Science Stuff:

Please help reform English libel law
Current UK libel laws are very bad for science and free speech.

Cool science stuff:

RCSB Protein data bank: Molecule of the Month in alphabetical order

The PCR Song It's the little things that keep you going...

NCBI's Entrez Gene My favourite starting point for finding out what is known about any gene of interest.

NCBI's PubMed Where to go to find pretty much all research published in the last 30 years or so (may go back a lot further now). All newly published research is quickly added to the site.

The Genetic Code - table of the DNA/RNA triplet codes for amino acids. This is how DNA codes for protein.

Another PCR song Disco frenzy :)

Recent visitors

Today I am mostly...

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cloning stuff :)
12th Nov 2009, 17:31  


bethmadethis says:

Oh my. What are you planning on cloning?

13th Nov 2009, 23:36

Spidermonkey says:

Green fluorescent protein tagged onto my favourite protein of the moment, CAR (constitutive androstane receptor). It's one of those proteins that senses toxins and hormones and stuff like that, then goes into the nucleus to activate the genes that deal with the downstream metabolism of whatever it has encountered.
If all goes according to plan I should end up with lots of little circles of DNA (bacterial plasmids) that I can introduce to cultured mammalian cell lines, and then I can treat them with different chemical compounds (such as environmental toxicants or drug components). If CAR binds to any of these compounds I should be able to see it accumulate in the nucleus of the cells (because of the green fluorescent protein), if there is no binding CAR should stay mostly in the cytoplasm.
If this doesn't work then I will have to go back to trying to make viruses to do the job for me, so I'm hoping it will work as virus work will take a lot more faffing about.

14th Nov 2009, 00:02

bethmadethis says:

of course..!

14th Nov 2009, 17:30