Red Filbert has attractive red leaves and produces small but very tasty nuts

by FilbertFox

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Yesterdays sky

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I haven't seen much sky today, I am lying on the sofa with what feels like the 'ears' of Spiderbaby
10th Oct 2006, 15:16  

paintist says:

what on earth are you on about woman...feeling the ears of spiderbaby....what?????

10th Oct 2006, 16:06

paintist says:

oops, I forgot to say what a splendid piccy that is.....very surreal .....

10th Oct 2006, 16:07

FilbertFox says:

Spiderbaby get 'ears' when she gets all dizzy and poorly - i have a sore throat in my ears - hense the ears of Spiderbaby.

10th Oct 2006, 16:11

paintist says:

you poorly? Sorry to hear that :-( standard advice, rest, keep warm and drink plenty of fluids, hope you feel better soon....

10th Oct 2006, 16:12

FilbertFox says:

tis only a cold, the whole department has it at the mo. thats the trouble with freshers, they bring us new germs

10th Oct 2006, 16:49

crickson says:

Gits. I have just recovered from a cold brought in by bloody students no doubt.

10th Oct 2006, 16:50

Dhamaka says:

it's an "A"!!!

10th Oct 2006, 16:51

FilbertFox says:

Dhamaka - I forgot to mention that this was inspired by your sky kisses

10th Oct 2006, 16:59

Dhamaka says:

aww...
thank you :D

but I think it's a sign. How often do you get sky writing that's the first letter of a friend's name...

10th Oct 2006, 17:10

FilbertFox says:

and of Daughter No. 1. Whom i have known longer!

10th Oct 2006, 17:13

Dhamaka says:

sorry to Daughter No. 1 whose name I didn't/don't know

10th Oct 2006, 17:14

FilbertFox says:

this is her name . All spekt right an all. Now that was truely a sign

10th Oct 2006, 17:18

Dhamaka says:

aha!
indeed...

10th Oct 2006, 20:00

I'm liking the droplets in the corner. Lovely photo.

I've found I've managed to avoid far more colds after I stopped working for a university.

How's the student projects coming along?

11th Oct 2006, 08:02

Dhamaka says:

is that where you experiment on the students, with them, or (less excitingly) they do their own stuff...

(wishful thinking here..)

11th Oct 2006, 08:30

FilbertFox says:

bloody students all they are good for is to give us all colds.

D - we experiment on students and/or with them!

RAB - just be given a cast off PCR machine, tried to get it working and it just says 'sub-ambient temperature failure'. Never seen a machine like it, wading through hand book to find out what to do

11th Oct 2006, 11:49

Spiderbaby says:

Oh dear. I've nearly got my ears too :( I hate germs :(

I think it looks like an H or perhaps an F for FF :)

11th Oct 2006, 13:55

Viv says:

hope you are better soon!

12th Oct 2006, 01:31

Never had a 'sub-ambient temperature faliure' before, but had my fair share of 'uniformity block faliures'. Trust me, replace all the reagents with water (use glycerol for the taq), and dope one of them (say a primer) with the PCR product. It makes things a lot easier. You do need to make up tons of PCR product beforehand though.

We used to use a robocycler for the students; the moving robotic arm was quite a good visual even if the machine itself was crap.

Get well soon!

13th Oct 2006, 07:31

FilbertFox says:

RAB - i have suggested this to the powers that be, they are determined to try it without cheating this year. If it all goes horribly wrong then i reckon cheating is the way forward - that science for you

13th Oct 2006, 08:35

JokerXL says:

This is an excellent picture FF, love it!

13th Oct 2006, 21:34

Oh, bugger. Well in my experience it's a good idea to repeatedly tell the students to not add more taq than it says in the protocol. I had so many problems in the 3 years I was a demonstrator because the students would assume that 1ul of enzyme wasn't enough and add 10 instead, resulting in failed PCRs (the one year we didn't cheat!) and not enough Taq to go around.

16th Oct 2006, 08:15

FilbertFox says:

we have diluted our taq 1 in 10 anyway, and we are giving them an eppendorf with 20ul in of which they need 15.

The fun starts today, they have an all day microbiology prac, and they have to find time to do their DNA extraction from their very own buccal cells - spit and boil - just what you want to see on a monday morning

16th Oct 2006, 09:29

For some reason I read that as buccal, spit and boil cells. I was about to say that boil-lansing in a student practical would be a bad idea...

Good luck! Hope it goes well!

17th Oct 2006, 02:34